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Biothema创亚化工(上海)有限公司

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Biothema创亚化工(上海)有限公司

BioThema is entirely focused on analytical applications of the firefly luciferase reaction whether it is detecting cells, measuring enzyme activity or something else. In this reaction luciferin is activated with ATP (adenosine triphosphate) and thereafter oxidized to oxyluciferin and CO2. The oxyluciferin is formed in an excited state and emits light when going to its ground state. The light is normally measured in what is called a luminometer.

创亚化工(上海)有限公司是一家致力于生命科学和生物技术领域的高科技企业。公司由在国内科研试剂领域有着十几年从业经验的专业技术团队和企业管理团队组建而成,专门从事以抗促销Beckman A63881的磁珠 现货,moltox  11-101.5等现货,transgenomic  706020/706025现货,Megazyme的大部分现货,LIST现

cAvanti 、tedpellaBioLegend、Polyplus、jena bioscience、Mattek、MRC-Holland、polysciences、enzo、novus、TOCRIS、CAYMAN、Neuro Probe、cyguns、eylabs、NIBSC、chondrex、lc laboratories、BTIetonbio

 

Biothema创亚化工(上海)有限公司

Biothema创亚化工(上海)有限公司

Biothema创亚化工(上海)有限公司

 

ATP+Luciferin+O2—Luciferase—>AMP+Pyrophosphate+Oxyluciferin+CO2+light

In assays of ATP the light emission may be essentially constant or decay rapidly. Thus there are stable light and flash type ATP reagents based on the firefly luciferase reaction. At low levels of luciferase a negligible proportion of the ATP is consumed per minute and the light can then be almost constant but also low. At high levels of luciferase it is inevitable that ATP is consumed resulting in a rapidly decreasing light but initially the peak light is high. Actually the peak height and the decay rate are both proportional to the luciferase activity. BioThema has developed three types of ATP reagents66:

ATP Eliminating Reagent

426 SEK

ATP degrading enzyme

Depending on with diluent is used when reconstitution ATP Eliminating Enzyme it is either used for degrading mammalian ATP and measuring bacterial ATP or for degrading extracellular ATP and measuring mammalian cells.

SKU: 71-101

 

 



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